HiTrap Capto ImpRes can serve as a universal column for routine work in a research lab. researching the mode of action of antimalarial drug primaquine.analysing the influence of a mitochondrial protein in mammalian cytosolic Fe-S repair.Since the resolution is similar to Sepharose High Performance, Capto ImpRes can be used in these applications as well, and there is no need to have both columns on hand.Ĭapto ImpRes resin has been used in diverse applications, including: HiTrap Capto ImpRes columns can be used for viscous samples as well as for other high-resolution IEX chromatography procedures with a broad range of samples. In this example, the IEX total run time is 30% shorter with HiTrap Capto Q ImpRes than a HiTrap Q HP, with an otherwise similar performing column.įor a larger image, click here. coli lysate, and allows them to be loaded at higher flow rates in a HiTrap Capto ImpRes than a HiTrap HP column. (Capto ImpRes resin has maximum flow rate of 300 cm/h vs 150 cm/h for Sepharose High Performance.) This allows more flexibility - even in the HiTrap format - for viscous samples such as E. HiTrap Capto ImpRes contains Capto ImpRes high-flow agarose resin, which has much better pressure/flow properties than Sepharose High Performance resin. Utilising a robust, modern and rigid resin - such as Capto Q ImpRes - reduces sample run time significantly by allowing high flow rate with low column backpressure, as seen in the example below. coli extract, can increase back pressure on the column and force you to reduce the flow rate, resulting in longer load times. That is the case when choosing a Capto ImpRes resin for use with viscous samples. While protein purification has become almost routine, new technology and materials always create opportunity to look for improvements. Ion exchange chromatography can be used in the first steps of protein purification for both native proteins and untagged recombinant proteins. The less time spent on purification, the more time you will have to focus on studying your protein. Today’s protein researcher is pressed to deliver results faster than ever before. In a recent webinar, we shared some tips for improving protein purity and recovery while performing IEX chromatography here, we focus on your time. Have you ever considered how a resin bead in your chromatography column can affect your ion exchange (IEX) chromatography run time?

Capto sp impres